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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #62603
Title: THE SEARCH FOR MOLECULAR MARKERS LINKED TO ALUMINUM TOLERANCE IN CEREALS

Author
item SOMERS, DARYL - UNIVERSITY OF MISSOURI
item BRIGGS, KEITH - UNIVERSITY OF ALBERTA
item BUTLER, ED - UNIVERSITY OF MISSOURI
item Gustafson, J

Submitted to: Food and Agriculture Organization International Atomic Energy Agency
Publication Type: Proceedings
Publication Acceptance Date: 6/19/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: We examined protein expression in an isogenic system of wheat (Triticum aestivum L.) in order to determine if proteins may be used as molecular markers for Al-tolerance. This system consisted of a dominant Al-tolerance gene from 'Maringa' (Al-tolerant) transferred to 'Katepwa' (Al-sensitive) which produced the near isoline 'Alikat' (Al-tolerant). Studies including L[35S]-methionine incorporation showed that protein synthesis increased in root tips of all three lines. 'Katepwa' showed a lack of protein accumulation in root tips relative to increased root tip dry weight with Al stress. This lack of protein accumulation was associated with an increase in proteinase activity in Al-sensitive 'Katepwa'. 'Maringa' and 'Alikat' showed normal, relative changes in root tip dry weight and protein content and no increase in proteinase activity following Al stress. Root tip polypeptide profiles showed that Al tolerance in 'Maringa', 'Alikat' and Al-tolerant F2's was a constitutive response; there were marginal quantitative changes in the polypeptide profile following Al stress. In contrast, 'Katepwa' showed numerous polypeptides that were either enhanced or reduced in quantity, following Al stress. We conclude that changes in gene expression may be deleterious to Al-tolerance in wheat and are associated with Al-sensitivity. This isogenic system is also being used for the isolation of DNA markers that co-segregate with the Al-tolerance phenotype. Two RFLP markers were recently isolated by Dr. C. Riede (North Dakota State University, USA) that are located on chromosome 4DL. We are mapping these markers in an F2 population derived from 'Katepwa' x 'Alikat'.