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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #62607
Title: THE PHYSICAL LOCATION OF A-AMY-1 GENE IN SECALE CEREALE L.

Author
item BUTNARU, GALLIA - AGRON INST OF ROMANIA
item Gustafson, J

Submitted to: Agronomy Institute of Romania Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The present study was carried out to estimate the location and the physical distance of the alpha-amylase-1 (a-AMY-1) gene in Secale cereal L., for placement on the cytological map. Techniques for slides preparation included a modified protoplast method and meristem squash. The probe for in situ hybridization was a 1.5 Kb a-AMY-1 clone. The chromosome arm ratio oand physical location of the a-AMY-1 gene was measured on 5-15 slides. Generally, hybridization occurred on only one chromatid. The a-amylase genes have been mapped genetically on 3 chromosomes: on the long arm of 1R and 7R, and on the short arm of 6R. The hybridization sites on the two varieties Blanco and Harkov were slightly different. The best detection, 3.8% from 1103 observed metaphases, was on Blanco. The mean location, as a percent distance from the centromere to the telomere, was 43.24 +/ 3.52% on 1RL; 50.81 +/ 1.45% for 6RS and 50.79 +/ 1.88% on the 7RL chromosome. The physical mapping analysis of a-AMY-1 showed a large variation among the varieties.