BIOSYNTHESIS OF FUMONISIN AND AAL DERIVATIVES BY ALTERNARIA AND FUSARIUM IN LABORATORY CULTURE

Authors: MIROCHA, C - UNIV OF MINNESOTA; CHEN, JUNPING - UNIV OF MINNESOTA; XIE, WEIPING - UNIV OF MINNESOTA; XU, YICHUN - UNIV OF MINNESOTA; Abbas, Hamed; HOQQE, L - SASKATCHEWAN CANADA

Submitted to: American Chemical Society Symposium Series
Publication Type: Book / Chapter
Publication Acceptance Date: 3/27/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary: The fumonisins and AAL-toxin are structurally related compounds usually produced by Fusarium moniliforme and Alternaria alternata, respectively. There has been controversy over the presence of fumonisins in A. alternata. This study confirms the presence of the fumonisins, FB1, FB2 and FB3, in isolates of A. alternata, demonstrating that the biosynthetic pathway for synthesis of the fumonisins is present in A. alternata.

Technical Abstract: Cultures of Fusarium moniliforme and Alternaria alternata f. sp. lycopersici were grown in the laboratory and analyzed for various fumonisin derivatives. Analyses were made by continuous flow fast atom bombardment and ionspray mass spectrometry interfaced to microcapillary HPLC. Besides FB1, FB2 and FB3 derivatives, two isomers of the one-armed FB1 (protonated molecular ion at m/z 564) and two isomers of the one-armed FB2 (m/z 548) were found. Two different isolates of A. alternata when grown in culture yielded FB1, FB2 and FB3. One of them also yielded the one-armed FB1 which was identical to that metabolite found in Fusarium and naturally infected corn. FB1, FB2 and FB3 have been found in 2 different isolates of Alternaria alternata, both obtained from tomato.