Skip to main content
ARS Home » Research » Publications at this Location » Publication #63294
Title: COMPARISON OF ELISA AND HPLC METHODS FOR THE DETECTION AND QUANTIFICATION OF SALINOMYCIN IN CHICKEN LIVER TISSUE

Author
item Muldoon, Mark
item ELISSALDE, MARCEL - 6202-30-10
item Beier, Ross
item Stanker, Larry

Submitted to: American Chemical Society Symposium Series
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/3/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary: Salinomycin is one of the most widely used therapeutic drugs in the US poultry industry. Conventional analytical methods to detect this drug are sensitive but are complicated and very time consuming. A rapid and accurate analytical method would be valuable to producer and user groups as an effective management tool. We have developed a rapid enzyme-linked immunosorbent assay (ELISA) for the analysis of salinomycin in chicken liver tissue. The method was used to analyze salinomycin in tissues from chickens which were fed the drug. These results were compared to results obtained using conventional technology. Both of these methods gave similar results. The ELISA method was more sensitive than the conventional method.

Technical Abstract: Salinomycin is one of the most widely used coccidiostats in the US poultry industry. A rapid and accurate analytical method for this drug would be valuable to producers and users as an effective management tool. We have developed an enzyme-linked immunosorbent assay (ELISA) coupled to a simple aqueous extraction procedure for the analysis of salinomycin in chicken liver tissue. Recoveries from spiked liver homogenates were quantitative in the range from 5.0 to 0.05 ppm. The ELISA was used to monitor fractions from reversed-phase high performance liquid chromatography (HPLC) in order to characterize nonspecific matrix effects on the assay. Results from the analysis of incurred salinomycin residues in chicken livers, obtained by both the ELISA and HPLC methods, were highly correlated (P greater than 0.0001). The ELISA method could detect 20 ng of drug in a 100 microliter sample and has a limit of quantitation of 50 ppb in chicken liver tissue. This was more sensitive than the HPLC method.