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Title: COMPARATIVE EFFECTS OF BOVINE CYTOKINES ON CATTLE AND BISON PERIPHERAL BLOOD MONONUCLEAR CELL PROLIFERATION

Author
item Stevens, Mark
item Olsen, Steven
item CHEVILLE, NORMAN - IA STATE UNIV

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/14/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Proliferation of peripheral blood mononuclear cells (PBMC) from cattle and bison was measured following stimulation of PBMC with bovine cytokines. Bovine interleukin 1b (BoIL-1b), interleukin 2 (BoIL-2), or granulocyte-macrophage colony-stimulating factor (BoGM-CSF) at 100 to 0.1 U/ml were incubated for 48 h with PBMC alone or with PBMC and various mitogens. These included concanavalin A (Con A), phytohemagglutini (PHA), pokeweed mitogen (PWM), or Escherichia coli 055:B5 lipopolysaccharid (LPS) at 10 to 0.1 mg/ml. BoIL-2 alone, but not BoIL-1b and BoGM-CSF alone induced proliferation of bovine and bison PBMC in the absence of mitogens. mitogens except PWM were effective as activation signals which enabled bovi and bison PBMC to become responsive to bovine cytokines. In addition, BoIL- and BoIL-2, but not BoGM-CSF, enhanced proliferation of bovine and bison PBMC that was induced by Con A, PHA, or LPS. These results indicate that BoIL-1b and BoIL-2 stimulate bovine and bison PBMC proliferation in a simi manner whereas BoGM-CSF does not appear capable of stimulating either bovin or bison PBMC proliferation. In addition, the capacity of bovine and bison PBMC to respond to BoIL-2 without additional activation stimuli most likely resulted from constitutive expression of IL-2 receptors on the cells.