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Title: LYMPHOCYTE PROLIFERATION IN RESPONSE TO BRUCELLA ABORTUS RB51 AND 2308 PROTEINS IN RB51-VACCINATED OR 2308-INFECTED CATTLE

Author
item Stevens, Mark
item Olsen, Steven
item CHEVILLE, NORMAN - IA STATE UNIV

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/14/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Proliferation of superficial cervical lymph node lymphocytes in response to Brucella abortus strain RB51 (SRB51) or strain 2308 (S2308) proteins (106 to 18 and < 18 kDa) was measured in cattle at 12 weeks after vaccination with SRB51. In addition, proliferation of supramammary lymph node lymphocytes in response to the SRB51 and S2308 proteins was measured in S2308-infected cattle following either abortion or infection of the fetus with S2308. Cattle vaccinated with SRB51 or infected with S2308 had lymph node lymphocytes which proliferated most when incubated with 32, 27, 18, or < 18 kDa proteins of either SRB51 or S2308. Some S2308-infected cattle but no SRB51-vaccinated cattle had lymphocytes which proliferated in response to 80 and 49 kDa proteins of SRB51 and S2308. These results suggest that SRB51 and S2308 contain immunodominant proteins of similar molecular masses (32, 27, 18, < 18 kDa) as assessed by measuring lymphocyte proliferation to the proteins in cattle that have been vaccinated with SRB51 or infected with S2308. In addition, cattle vaccinated with SRB51 or infected with S2308 have lymphocytes which respond to most of the same S2308 proteins. Consequently, the SRB51 vaccine induces lymphocyte responses to certain S2308 proteins which are similar to those associated with infection with S2308, and perhaps these vaccine-induced responses have a role in protective immunity.