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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Ruminant Diseases and Immunology Research » Research » Publications at this Location » Publication #64465
Title: COMPARISON OF WEHI 164 CLONE 13 AND PK(15)-BASED BIOASSAYS FOR THE DETECTION OF PORCINE TNF

Author
item MAROUSHEK-BOURY, NANCY - IA STATE UNIV., AMES, IA
item Stabel, Thomas
item Kehrli Jr, Marcus
item TAYLOR, M - IA STATE UNIV., AMES, IA

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/14/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Murine cell lines have been widely used to detect tumor necrosis factor (TNF) by bioassay. In 1986, it was reported that a subclone of the murine WEHI 164 cell line, clone 13, was one hundred times more sensitive than the other murine cell line, L929, at detecting human and murine TNF. Recently, a new bioassay was described using a porcine cell line, PK(15) (ATCC CCL33) in the place of the more commonly used murine cells. The PK(15)-based bioassay was 100-1,000 times more sensitive than the L929-based assay when detecting porcine TNF. We compared the sensitivity of PK(15) and WEHI 164 clone 13 cells to human, murine, and porcine TNF-mediated lysis. Our data indicates that while the cell lines showed equivalent susceptibility to human TNF, the PK(15) cells are 200-fold less sensitive to murine TNF than are WEHI clone 13 cells, but 16 to 18 times more sensitive to porcine TNF. While the murine WEHI 164 clone 13 cells are a great improvement over L929 cells at detecting porcine TNF, the PK(15)-based bioassay was even more sensitive.