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Title: DISTRIBUTION OF RETROTRANSPOSON MAGGY IN PYRICULARIA SPECIES

Author
item TOSA, YUKIO - KOCHI UNIV JAPAN
item NAKAYASHIKI, HITOSHI - KOBE UNIV JAPAN
item HYODO, HIDEYUKI - KOCHI UNIV JAPAN
item MAYAMA, SHIGEYUKI - KOBE UNIV JAPAN
item KATO, HAJIME - KOBE UNIV JAPAN
item Leong, Sally

Submitted to: United States Japan Seminar Series
Publication Type: Abstract Only
Publication Acceptance Date: 8/15/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Pyricularia species are isolated from many monocot species. Several researchers have attempted to group these isolates by examining traits such as morphology, host range, and sexual compatibility. Although at least five subgroups (isolates from mioga, crabgrass, Manchurian wild rice, finger millet, and rice) could be clearly discriminated, there remained many isolates whose grouping was ambiguous. MAGGY is a retrotransposon found in the genomic DNA of Pyricularia species. It is 5.6 kb in size and flanked by 253 bp LTRs (Long Terminal Repeats). Its internal region shows homology at the peptide level to reverse transcriptases of the Gypsy class of retrotransposons. In the present study, the distribution of MAGGY in Pyricularia isolates from various hosts was investigated to reveal their phylogenetical relationships. Genomic DNA was extracted from 48 Pyricularia isolates from various hosts, digested with PstI, and hybridized dwith the MAGGY probe. MAGGY was present in a high copy number (at least 35-45 copies) in isolates from rice, foxtail millet (Setaria italica), and green bristlegrass (S. viridis), but absent in those from wheat, finger millet, goosegrass, crabgrass, Digitaria horizontalis, and mioga. These results suggest that isolates from rice are phylogenetically close to those from Setaria species. The present results also suggest that MAGGY will be a good probe for fingerprinting of rice and Setaria isolates.