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Title: PROTON AMINO ACID SYMPORTERS IN ARABIDOPSIS: STRUCTURE, FUNCTION, AND MOLECULAR CLONING

Author
item CHEN, LISHAN - PLANT BIO UNIV ILL URBANA
item BUSH, DANIEL

Submitted to: Membrane Biophysics Advances in Coupled Membrane Transporters Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 10/17/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: While plants are photoautotrophic organisms, they contain many heterotrophic tissue systems that must import sugars and amino acids to support growth and development. This resource redistribution process is known as assimilate partitioning and it is a fundamental activity that allows higher plants to function as multicellular organisms. Previous work in our lab identified four amino acid transport systems based on biochemical analysis of their transport properties in purified membrane vesicles. These are secondary active transporters that couple flux to the proton electrochemical potential difference across the plant cell plasma membrane. Subsequently, we cloned one of these carriers, neutral amino acid transport system II (NAT2), by functional complementation of a yeast transport mutant with Arabidopsis cDNA library. In the results presented here, we report on recent work describing the structure of the transport protein.