Author
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CHIOU, TZYY-JEN - PLANT BIO UNIV ILL URBANA |
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BUSH, DANIEL |
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Submitted to: Membrane Biophysics Advances in Coupled Membrane Transporters Symposium
Publication Type: Abstract Only Publication Acceptance Date: 10/17/1995 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Sugar transport within cells and between organs is a fundamental activity in all multicellular organisms. Recently, several plant genes have been cloned that are members of the sugar transporter subgroup of the major facilitator superfamily (MFS) of transporters. Members of this gene family are found in both eukaryotic and prakaryotic organisms, and are believed to be derived from a common, primordial transport system. In spite of convincing sequence similarity with known sugar transporters, only a few of the plant clone have been described with respect to transport function. In the results presented here, we report on the expression and surprising membrane localization of one of these porters found in sugar beet. Antibodies directed against small peptides representing the N- and C-terminal domains of the expressed protein of our clone, cDNA1, were produced as tools to study this low abundance membrane protein. In transgenic yeast, an additional 40 kDA polypeptide was observed in western bolt analysis of the microsomal membrane fraction of cDNA-1 transformed cells versus the non-transformed controls. This transporter was also examined in sugar beet and transgenic tobacco. Western analysis identified the cDNA1 protein in the tongdest membrane. This is only the second report of a sugar transporter gene product targeted to an endomembrane compartment. |
