Author
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Dobrinsky, John |
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Submitted to: Theriogenology
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 10/15/1996 Publication Date: N/A Citation: N/A Interpretive Summary: Considerable progress has been made in the improvement and simplification of cryopreservation procedures routinely used in embryo transfer programs. Conventional slow-rate, programmable freezing and vitrification of embryos have given veterinarians, scientists and producers alternatives in their herd reproduction practices, however, pregnancy rates after cryopreservation are not equivalent to fresh embryo transfer. No matter ho embryos are cryopreserved, some always die, and that can be costly to the producer. This paper addresses intracellular damage to the cytoarchitecture of cryopreserved embryos and attempts to detour this damage by utilizing cytoskeletal stabilization prior to cryopreservation. This approach documents cellular damage from cryopreservation and provides useful, non- empirical information for understanding cellular sensitivities to cryopreservation. It will lead to improved protocols for embryo cryopreservation and a better understanding of domestic animal embryology. Technical Abstract: Considerable progress has been made in the improvement and simplification of cryopreservation procedures routinely used in embryo transfer programs. Conventional slow-rate, programmable freezing and vitrification of embryos have given veterinarians, scientists and producers alternatives in their herd reproduction practices, however, pregnancy rates after cryopreservation are not equivalent to fresh embryo transfer. No matter ho embryos are cryopreserved, some always die, and that can be costly to the producer. Documenting cellular damage during or after cryopreservation would provide useful, non-empirical information for understanding cellular sensitivities to cryopreservation and will lead to improved protocols for embryo cryopreservation and a better understanding of domestic animal embryology. This paper discusses the current progress in embryo cryopreservation, and will address intracellular damage to the cytoarchitecture of cryopreserved embryos and attempts to detour this damage by utilizing cytoskeletal stabilization prior to cryopreservation. |
