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Title: PATTERN OF GRANULOSA CELL PROLIFERATION DURING ANTRAL FOLLICULAR GROWTH IN MEISHAN GILTS

Author
item FRICKE, PAUL - UNIV OF NORTH DAKOTA
item FORD, JOHNY
item REYNOLDS, L - UNIV OF NORTH DAKOTA
item REDMER, D - UNIV OF NORTH DAKOTA

Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: 2/22/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Meishan gilts were ovariectomized on Day 15 to 19 of the estrous cycle or 24 to 30 h after observed estrus (post-LH, PLH). Two h before ovariectomy, each gilt received an iv injection of bromodeoxyuridine (BrdU; a thymidine analog). BrdU was immunolocalized in histological section of follicles greather than or equal to 3 mm in diameter, and the labeling index (LI; % of nuclei staining for BrdU) within the basal, middle, and antral thirds o the granulosa was quantified for each follicle. Area of Granulosa Day 15 Day 16 Day 17 Day 18 Basal LI (%) 5.7 3.8 2.4 1.3 Middle LI (%) 11.4 8.8 4.6 5.0 Antral LI (%) 7.7 8.7 8.7 9.1 Antral volume and numbers of granulosa cells also were determined for each follicle. Follicles from Day 19 and PLH gilts had few and no labeled granulosa cells, respectively. Mean follicular diameter (mm) increased linearly (p < .01) from 4.0 at Day 15 to 7.9 at PLH. Antral volume (mm3) increased linearly (p<.01) from 12.9 at Day 15 to 100.5 at Day 19, resulting in 2.8 volume doublings and a doubling time of 1.4 days. Number of granulosa cells/follicle (x 1,000,000) increased linearly (p<.01) from 4.2 at Day 15 to 15.4 at PLH, resulting in 1.5 cell doublings and a doubling time of 3.3 days. Thus, as follicles matured, cell proliferation decreased in the basal and middle but not the antral area of the granulosa. In addition, follicular growth resulted primarily from antral expansion.