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ARS Home » Research » Publications at this Location » Publication #65974
Title: RAPID PURIFICATION OF FUMONISINS B3 AND B4 WITH SOLID PHASE EXTRACTION COLUMNS

Author
item Poling, Stephen
item Plattner, Ronald

Submitted to: ARS Workshop on Fusarium Toxins Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/26/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: A simple method was developed to isolate fumonisin B3 and B4 (FB3 and FB4) from cultures of Fusarium moniliforme. A novel strain of F. moniliforme which only produces FB3 and FB4 but no FBl or FB2 was used. Extracts from the culture material were loaded onto NH2 cartridges and the fumonisins eluted with 5% HOAc/MeOH, which was diluted with 1.5 volumes of water and loaded onto a tCl8 cartridge. The fumonisins were eluted with increasing amounts of CH3CN in H20. Complete separation was obtained between the FB3 fraction (FB3 plus about 20% as much FA3 and a trace of FC3) and the FB4 fraction (FB4 plus about 11 and 18% as much FA4 and FC4, respectively). The purities were measured by three methods: fluorescence detection of the OPA derivatives, evaporative light scattering detection of the underivatized fumonisins, or by electrospray-MS and the results compared. The fractions were a least 90% fumonisins and probably closer to 95% pure. A 277-mg FB3 fraction and a 62-mg FB4 fraction were obtained from the combination of a 10-g NH2 and a 10-g tCl8 cartridge. The method should be useful for providing FB3 and FB4 for toxicity studies or for further purification by semi-preparative HPLC. It should also be useful for working with radio-labeled material.