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ARS Home » Southeast Area » Raleigh, North Carolina » Plant Science Research » Research » Publications at this Location » Publication #67228
Title: DESYNAPTIC (DY) MUTANT REDUCES CROSSOVER RECOVERY ON CHROMOSOME 1L

Author
item WILLIAMS, CLAIRE - TEXAS A&M UNIVERSITY
item Stuber, Charles
item GOODMAN, MAJOR - NORTH CAROLINA STATE UNIV

Submitted to: Maize Genetics Cooperation Newsletter
Publication Type: Abstract Only
Publication Acceptance Date: 1/18/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Desynaptic is a meiotic mutant first reported by Nelson and Clary (J. Hered. 43:205-210, 1952). Cytogenetical analysis shows dy mutants do not display orderly disjunction during anaphase I, after crossing-over has occurred (Maguire, Chromosoma 65:173-183, 1978). Mutant plants can be phenotyped easily by a reduced proportion (65- 75%) of normal pollen and this can be verified with pachytene analysis. Cytogenetic analysis of asynaptic, another maize meiotic mutant, did not corroborate marker-based approaches to recovering meiotic products so we tested the hypothesis that dy is a gene which modifies recombination frequencies. Mutant dy plants from stock verified by Maguire et al., (Genome 34:879-887, 1991) were crossed to a chromosome 1L rare-allele stock and a single F1 parent was selfed to produce 200 F2 plants segregating for dy. Mutant and wild-type F2 parents which were segregating for marker loci were then selfed to produce F3 offspring. Linkage distances were compared between a mutant dy/dy F2 family and a wild-type Dy/dy F2 family composed of 100 offspring each. Preliminary data show that the wild-type offspring has a total map distance comparable to our previous study (72.3 cM for the amp1-gdh1 interval on chromosome 1L) but the dy/dy mutant offspring showed a total of 46.9 cM for the same interval.