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ARS Home » Midwest Area » East Lansing, Michigan » Sugarbeet and Bean Research » Research » Publications at this Location » Publication #67571
Title: MANIPULATION OF SOMATIC EMBRYO AND SHOOT NUMBERS IN SUGARBEET CALLUS WITH BENZYLADENINE, ABSCISIC ACID, AND TRIIODOBENZOIC ACID

Author
item Saunders, Joseph
item TSAI, CHIA - MICHIGAN STATE UNIVERSITY

Submitted to: Congress on In Vitro Biology
Publication Type: Abstract Only
Publication Acceptance Date: 5/20/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Sugarbeet (Beta vulgaris L.) clone REL-2, a much more prolific shoot and somatic embryo producer from hormone autonomous leaf disc callus than REL-1, one of its parents, produces leaf disc callus with somatic embryos in the absence of growth regulators. Suspension cultures of both clones plated onto all combinations of 0, 0.1, 0.3 and 1.0 mg/L 6-benzyladenine (BA) and 0, 0.02, 0.2, and 2.0 mg/L abscisic acid (ABA) produced progressively fewer somatic embryos but more shoots at progressively higher BA levels (per plate, maximum somatic embryo number was 6 and 45, and shoot number 65 and 70, for REL-1 and REL-2 respectively). For some BA levels, the presence of ABA significantly improved somatic embryo and/or shoot numbers, up to twenty-fold. Suspensions plated on all combinations of the above levels of ABA and 0, 0.1, 0.3, 1.0, 3.0 and 10.0 mg/L triiodobenzoic acid (TIBA) at 1.0 mg/L BA produced more somatic embryos and shoots at 2.0 mg/L ABA over an optimal range of TIBA levels only for REL-1. Use of these clones for transgenic efforts or somatic cell selection should be facilitated by this research.