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ARS Home » Research » Publications at this Location » Publication #68066
Title: APPLICATIONS OF CAPILLARY ISOELECTRIC FOCUSING TO ANALYTICAL BIOTECHNOLOGY ASSAYS

Author
item HUANG, TUNG-LIANG - BECKMAN INSTRUMENTS
item LIU, MING-SUN - BECKMAN INSTRUMENTS
item Richards, Mark
item SHIEH, PAUL - BECKMAN INSTRUMENTS
item COOKE, NELSON - BECKMAN INSTRUMENTS
item CHEN, ALBERT - BECKMAN INSTRUMENTS

Submitted to: Capillary Electrophoresis Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 4/5/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Due to the speed, minute sample requirement, on-line detection, automated data processing, and ease of labor, capillary isoelectric focusing (CIEF) is a fully automated instrumental approach for resolving proteins based on their pI differences. The method involves first focusing the samples in the capillary and subsequently mobilizing the sample zones past the on-column detector. The use of constant pressure as the mobilization force makes all the focused zones pass the detector with the same speed, leading to a highly linear pH gradient and good quantitation capability. Coupled with laser induced fluorescence (LIF) detection, the detection limit of the CIEF method can be increased by three orders of magnitude, compared to UV detection. This study demonstrates the analysis of biomolecules as applied to analytical biochemistry. The separation performance in terms of reproducibility, quantitation, and detection limit will be discussed.