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Title: HOST RANGE OF THREE CYTOPLASMIC POLYHEDROSIS VIRUSES IN INSECT CELL CULTURES

Author
item Lynn, Dwight
item SHAPIRO, MARTIN - 1275-21-00

Submitted to: In Vitro Cellular And Developmental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 4/27/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: While baculoviruses (particularly the nuclear polyhedrosis viruses, NPVs) have been extensively studied in recent years as potential biopesticides, the cytoplasmic polyhedrosis viruses (CPVs) have been largely ignored. In the process of screening a new cell line from the white marked tussock moth, Orgyia leucostigma (IPLB-OlE505s) to its homologous baculovirus (OlNPV), a CPV was observed in cells of the gypsy moth (Lymantria dispar) which were inoculated with the OlNPV as a control. This virus is designated OlCPV. During routine bioassays of other NPVs in cabbage looper larvae (Trichoplusia ni), another CPV (designated TnCPV) was observed to be infecting the insects, both in untreated controls and those fed NPV. We have isolated these two viruses and used them with a third from the gypsy moth (LdCPV) to determine their respective in vitro host ranges. These viruses were exposed to various lepidopteran cell lines which were examined for the presence of polyhedra in the cytoplasm. To date, screening of TnCPV reveals it is capable of infecting 15 different cell lines from 9 species of Lepidoptera. LdCPV (thus far only screened against gypsy moth cells) infects two embryonic and an ovarian cell line but does not infect a fat body cell line. Due to the low level of infection by OlCPV, we have had insufficient material to do an extensive study, but it does infect the gypsy moth fat body line which is refractory to its homologous CPV. Additional studies will be performed to further characterize these viruses.