Author
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Bietz, Jerold |
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WAGA, J - PLANT BREEDING & ACCLIMAT |
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Submitted to: American Association of Cereal Chemists Meetings
Publication Type: Abstract Only Publication Acceptance Date: 9/19/1996 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Wheat gliadins are coded by genes at six major complex loci on homoeologous chromosome groups 1 and 6. The genes in each locus are closely linked, causing co-expression of proteins they code. Alternate groups of genes, or alleles, occur in different cultivars. Thus, the genotype of any variety can be denoted by a formula identifying alleles at each locus. This has most often been done by visual interpretation of acid gel electrophoresis patterns. Specific gliadin blocks also relate to quality characteristics, so selection based on gliadin-coding alleles is used during wheat breeding, especially in eastern Europe. Reversed-phase high-performance liquid chromatography (RP-HPLC) and capillary electrophoresis (CE) now offer many advantages over gel electrophoresis for gliadin analysis. RP-HPLC and CE have not, however, yet been used to characterize gliadin blocks. We therefore compared gliadin compositions of wheat genotypes with known gliadin alleles by gel electrophoresis, RP-HPLC, and CE. Comparison of genotypes differing by only one allele enabled identification of proteins belonging to specific blocks. Results show that RP-HPLC and CE are high-resolution and complementary alternatives to gel electrophoresis. RP-HPLC and CE confirm knowledge of alleles coding gliadins and can show heterogeneity not readily apparent by gel electrophoresis. Results suggest that block structure analyses by RP-HPLC and CE can become valuable tools for wheat breeding and in genetic studies. |
