Author
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Smith, Eugene |
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Cheng, Hans |
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Submitted to: Poultry Science Meeting
Publication Type: Abstract Only Publication Acceptance Date: 7/8/1996 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Through genetic linkage analysis, localization of functional genes (Type I markers) will provide a framework for comparing the conservation of blocks of genes throughout evolution. Ultimately, identification of syntenic groups in chickens with those of mammalian species having more abundant markers will facilitate gene identification and marker assisted selection. We have used the polymerase chain reaction to amplify across introns or the 3' untranslated region of chicken genes homologous with those from mammalian species that are mapped on their respective chromosomes. Cloning and nucleotide sequence analysis of PCR products was used to identify polymorphisms, i.e., nucleotide substitutions or deletions. The JF allele was identified by preferential amplification of specific alleles (PASA) or differences in size of PCR products. The segregation of the Jungle Fowl (JF) allele among 52 backcross progeny of the East Lansing JF X White Leghorn cross was detected in ethidium bromide stained agarose gels. Using this approach, we have mapped 21 known genes to date. Although synteny has yet to be established, both the retinoblastoma oncogene (RB1) and the lysosomal membrane glycoprotein (LAMP1), gene are on chicken chromosome 1 and the long arm of human chromosome 13. Among noted polymorphisms, nucleotide transitions were more frequently observed than transversions. Polymorphisms were not detected among 14 cloned PCR products (250-400 base pairs) amplified from other candidate genes. |
