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Submitted to: Rice Technical Working Group Meeting Proceedings
Publication Type: Proceedings Publication Acceptance Date: 2/1/1996 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: We have developed a set of 302 Recombinant Inbred Lines (RILs) 'Lemont'/'Teqing' for use as a permanent mapping population whereby costly molecular data can be collected once then used to locate QTLs for any character subsequently measured on the inbred progeny lines. When completed and verified, we will be publicly sharing molecular data on 120 RFLPs via RiceGenes. Meanwhile, we are gathering replicated, multi-year field data o the RILs and making small amounts of seed available to others. Transgressive segregation has been observed for each character measured to date, including characters where the two parents are not apparently different. We suggest screening 10% of the RILs as well as the parental lines when evaluating the population's capacity for providing specific mapping data. This population has more lines and appears to be less skewed than other available mapping populations, thus allowing identification of genes with smaller effect. Lessons learned while developing this mapping population include: a) select a widely compatible parent if possible, b) avoid environments that increase outcrossing (i.e., Puerto Rico), c) most phenotypic variance observed in F6 and later generations was attributable to mixture (i.e. birds) rather than heterozygosity - replication is imperative and panicle bagging may be limited to highly sterile lines, d) analyze QTL data statistically, but interpret it from a biological and technical viewpoint, e) skewing and natural selection will occur - document what you can and consider it when interpreting data, f) mapping multiple traits simultaneously can elucidate relationships between them, g) take advantage of unexpected segregation, h) use Dr. Susan McCouch's "Anchor" probes if possible. |
