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ARS Home » Midwest Area » Columbus, Ohio » Soil Drainage Research » Research » Publications at this Location » Publication #69573
Title: USING AFLP MARKERS TO DETERMINE THE CONTRIBUTION OF PARENTAL GENOMES DURINGRECURRENT SELECTION

Author
item Vantoai, Tara
item PENG, JIQING - THE OHIO STATE UNIVERSITY
item ST MARTIN, STEVE - THE OHIO STATE UNIVERSITY

Submitted to: Soybean Genetics Newsletter
Publication Type: Research Notes
Publication Acceptance Date: 4/5/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The AFLP technique is a very efficient DNA marker technique. Using a single primer pair, we were able to identify 14 polymorphic bands in a soybean breeding population selected recurrently. Since Taq polymerase is expensive, the high number of polymorphism detected makes this technique more cost efficient than the RAPD technique. With one single primer pair we were able to show that the contribution of the two adapted parental genomes remains essentially unchanged after two cycles of recurrent selection and that up to 25 % of the gene pool of the C0 and C1 populations were still derived from the plant introduction genomes.