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Title: AMPLIFICATION OF DNA FROM YELLOW VINE AFFECTED WATERMELON BY POLYMERASE CHAIN REACTION

Author
item AVILA, F - UNIVERSITY OF NEBRASKA
item Bruton, Benny
item SHERWOOD, J - OKLAHOMA STATE UNIVERSITY
item FLETCHER, J - OKLAHOMA STATE UNIVERSITY
item Pair, Sammy

Submitted to: American Phytopathological Society
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Yellow vine has caused serious losses of watermelon in central Texas and Oklahoma since 1991. The PCR with DNA from asymptomatic and symptomatic plants was conducted using primers known to amplify either the 16S rDNA of the bacterialike organism that causes citrus greening, or the 16S rDNA of both prokaryotes and chloroplasts. With the first set of primers, a 153 bp pfragment was consistently amplified from the DNA of plants with yellow vin symptoms, but not from the DNA of asymptomatic plants. The 153 bp fragment has a similarity (84% identity) to a segment of the gene encoding threonine in Escherichia coli. With the second set of primers, a 1500 bp DNA band was amplified from the DNA of both symptomatic and asymptomatic plants. Digestion of the 1500 bp with EcoR1 and Bcl1 showed that the band from yellow vine symptomatic plants was composed of two species. One species represents chloroplast DNA. The other species, which may represent prokaryotic DNA associated with yellow vine, is currently being resolved.