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ARS Home » Research » Publications at this Location » Publication #69662
Title: RAPID IDENTIFICATION AND DETECTION OF BROWN STEM ROT PATHOGEN IN SOYBEAN PLANTS

Author
item CHEN, WEIDONG - UNIV OF ILLINOIS
item Gray, Lynn
item GRAU, C - UNIV OF WISCONSIN

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 7/21/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: More than eighty strains of Phialophora gregata, the causal agent of soybean brown stem rot (BSR) collected from five states, were compared with other fungi associated with BSR by examining variations of nuclear rDNA. The strains of P. gregata were distinct from other fungi. Based on variations at the DNA sequences of the internal transcribed spacer, we designed two PCR primers specifically for P. gregata. The specific primers can be used to unambiguously differentiate P. gregata from other fungi and allow us to detect P. gregata in infected soybean plants. By using this procedure, P. gregata in greenhouse inoculated plants can be detected and confirmed within hours. The fungus was also detected in field-collected diseased stems. This rapid detection process can be used to detect P. gregata from field plants and used in soybean breeding programs for selecting resistance to BSR.