Author
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ZIPRIN, RICHARD |
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Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 7/10/1996 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Previous studies at this laboratory have shown that Salmonella enteritidis-immune lymphokine promotes the migration of heterophils into the chicken peritoneum in response to SE challenge. Others have shown in rodents that migration of neutrophils is partly induced by epithelial cell products produced when the cells interact with salmonellae. The present work compared the accumulation of intraperitoneal heterophils in day of hatch chicks following treatment with SE-immune lymphokines and challenge with different strains of SE. One strain was a wild type, another was an invasion capable avirulent vaccine strain (designed for oral vaccination), and a third contained mutations that altered invasiveness. Chicks received IP treatment with SE-immune lymphokine followed 1 hr later by IP challenge with one of the following SE strains: X3895, the delta-cya-12 delta-crp-11 derivative x4357, the InA::kan derivative X4420, or SE (890034-3). All work was done in a biosafety level 3 facility. Heterophils were recovered by peritoneal lavage. Total cell counts were determined and the heterophils were counted directly to determine the concentration of heterophils in the recovered lavage fluid. Heterophil concentrations increased in response to each SE strain; however, there was a low response to the invasionless strain. The difference was statistically significant. This diminished heterophil response to challenge with invasionless salmonellae supports the existing evidence that the initial defensive reaction occurs at the earliest stages of Salmonella-host interaction. |
