Skip to main content
ARS Home » Research » Publications at this Location » Publication #70264
Title: ANALYSIS OF GENETICALLY MODIFIED CANOLA VARIETIES BY ATMOSPHERIC PRESSURE CHEMICAL IONIZATION MASS SPECTROMETRIC AND FLAME IONIZATION DETECTION

Author
item Neff, William
item Byrdwell, William

Submitted to: Annual Meeting and Expo of the American Oil Chemists' Society
Publication Type: Abstract Only
Publication Acceptance Date: 4/28/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Canola oil triacylglycerols from genetically modified canola lines were conclusively identified by reversed-phase high performance liquid chromatography (RP-HPLC) coupled with atmospheric pressure chemical ionization mass spectrometric (APCI-MS) detection. APCI-MS is a soft ionization technique, which gave simple spectra for triacylglycerols. Spectral identification of the triacylglycerols was based on the diacylglycerol fragments and on the protonated molecular ion [M+H]**+, except trisaturates which gave no [M+H]**+. Triacylglycerols were identified and quantitated in normal, high stearate and high laurate canola varieties by a RP- HPLC/APCI-MS technique. The LC/APCI-MS identification of canola oil triacylglycerols allowed their quantitation by reversed-phase HPLC coupled with a commercially available flame ionization detector (FID). There was agreement between the fatty acid composition obtained by LC/APCI-MS and LC-FID. However, the triacylglycerol resolution obtained by LC/APCI-MS was superior to LC-FID in the qualitative identification of triacylglycerols present in amounts below one percent. The LC/APCI-MS technique gave better resolution of, and quantitation of triacylglycerols in the canola oils than the LC/FID. However, the LC/FID system gave satisfactory analyses suitable for many research programs, like the development of genetically modified canola varieties with oils of improved oxidative stability.