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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #70705
Title: APPLICATION OF A TEST FOR DIFFERENTIATION OF STRAINS OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS BY DIRECT EXAMINATION OF DIAGNOSTIC SAMPLES

Author
item Mengeling, William
item Lager, Kelly
item Vorwald, Ann
item ROOF, MICHAEL - NOBL LABORATORIES INC
item Wesley, Ronald

Submitted to: American Association of Veterinary Laboratory Diagnosticians
Publication Type: Proceedings
Publication Acceptance Date: 10/18/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: We recently developed a test for differentiating strains of porcine reproductive and respiratory syndrome (PRRS) virus (PRRSV). The test has great application in epizootiological studies to trace the origin of PRRSV involved in new outbreaks of the disease. By identifying the source, further spread of the virus can often be prevented. In some cases test results are urgently needed, however, they are sometimes delayed by the requirement to first isolate PRRSV from diagnostic samples (currently the first step in the test procedure). In the study reported here we investigated the possibility of avoiding virus isolation by applying the test directly to tissues collected from pigs affected by the disease. Preliminary work described in this report has indicated virus isolation is not always needed. Research is continuing to determine the relative reliability of applying the test directly to tissue samples versus applying the test only after virus isolation from the same samples.