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Title: AN ANTIBODY TO GP82 WHICH INHIBITS MAREK'S DISEASE VIRUS PLAQUE FORMATION IN TISSUE CULTURE

Author
item WU, PING - MICHIGAN STATE UNIVERSITY
item REED, WILLIE - MICHIGAN STATE UNIVERSITY
item Lee, Lucy

Submitted to: International Marek's Disease Symposium Abstracts and Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/7/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: A TrpEgp82 fusion protein expression vector (pATHgp82) was constructed by cloning a 1,079bp fragment of gp82 gene into pATH. The partially purified trpEgp82 fusion protein was used as an antigen to develop polyclonal and monoclonal antibodies. Three clones of hybridomas secreting monoclonal antibodies 2.1 (Mab2.1), 3.7 (Mab3.7), and 5.7 (Mab5.7); and one rabbit antiserum were produced. All of the antibodies reacted with natural or denatured forms of gp82 protein in CEF cells infected with serotype 1 MDV by immunoprecipitation, Western blot assay, and indirect immunofluorescence assay. These antibodies only recognize MDV-1 gp82 protein, with no detectable reaction to both serotype 2 and serotype 3 MDVs by the previously mentioned three methods. Furthermore, the rabbit antiserum appears to inhibit the plaque formation in in vitro tissue cultures.