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Title: MAREK'S DISEASE VIRUS GENE ENCODING MAJOR CAPSID ANTIGEN HOMOLOGOUS TO HERPES SIMPLEX VIRUS

Author
item SUI, DEXIN - MICHIGAN STATE UNIVERSITY
item WU, PING - MICHIGAN STATE UNIVERSITY
item Lee, Lucy
item KUNG, HSING-JIEN - CASE WESTERN RESERVE

Submitted to: International Marek's Disease Symposium Abstracts and Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/7/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Nucleotide sequence analysis of Marek's disease virus genome revealed an open reading frame homologous to UL19 of Herpes Simplex virus (HSV) encoding the major capsid protein. The MDV UL19h mapped in the unique long region of the viral genome on the BamHI-C and BamHI-F fragments. A 350 bp junction fragment between BamHI-C and -F fragments was identified as the C-terminal portion of the MCP ORF by amplification of GA genomic DNA using polymerase chain reaction (PCR). Therefore, the complete MDV MCP gene, is a 4,053-base-pair fragment of the DNA encoding 1,351 amino acids with a Mr of 150,691 dalton. MDV MCP showed extensive homology to six distantly related herpesviruses, equine herpesvirus (EHV), Pseudorabies Virus (PRV), varicella-zoster virus (VZV), herpes simplex virus type 1 (HSV-1), Epstein-Barr virus (EBV), and human cytomegalvirus (HCMV).