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ARS Home » Research » Publications at this Location » Publication #71022
Title: DIFFERENCES IN GAP JUNCTION INTERCELLULAR COMMUNICATION BETWEEN CELLS INFECTED WITH EITHER ATTENUATED OR ONCOGENIC MAREK'S DISEASE VIRUS

Author
item HOLLAND, MARGO - MICHIGAN STATE UNIVERSITY
item Silva, Robert
item TROSKO, J - MICHIGAN STATE UNIVERSITY
item WILSON, M - MICHIGAN STATE UNIVERSITY

Submitted to: International Marek's Disease Symposium Abstracts and Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/7/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: In an effort to examine cellular and Marek's disease virus (MDV) interactions, we determined the effect of both oncogenic and attenuated MDV on gap junctional intercellular communication (GJIC) of chick embryo fibroblasts (CEF). There is a strong correlation between dysfunctional GJIC during the etiology of cancer. To identify alterations in GJIC, scrape loading dye assays, fluorescent recovery after photobleaching, and pre-loading dye assays were performed on MDV-infected CEFs. Each procedure relies on the passage of different dyes through gap junctions between cells if GJIC is functional. However, alterations in GJIC impedes the dye from spreading between adjacent cells. GJIC was inhibited in oncogenic MDV-infected CEFs but unaltered in attenuated and uninfected CEFs. This is the first demonstration of an oncogenic herpesvirus altering GJIC.