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ARS Home » Research » Publications at this Location » Publication #71057
Title: DNA SEQUENCE ANALYSIS OF MDV GENOMIC FRAGMENTS BAMHI-P2, I1, AND J REVEALS AN OPEN READING FRAME HOMOLOGOUS TO HERPES SIMPLEX VIRUS GENE ENCODING DNA-BINDING TEGUMENT PROTEIN

Author
item MAO, YAOPAN - MICHIGAN STATE UNIVERSITY
item WU, PING - MICHIGAN STATE UNIVERSITY
item Lee, Lucy

Submitted to: International Marek's Disease Symposium Abstracts and Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/7/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: DNA sequencing of the Marek's disease virus (MDV) genomic fragments BamHI-P2, I1, J and their overlapping EcoRI-E fragment were determined and they constitute one large open reading frame (ORF) of approximately 9.5 kilobases encoding a protein with a calculated molecular weight of 352 kilodaltons. Analysis of the ORF reveals that it is homologous to the UL36 gene of the herpes simplex virus (HSV) encoding ICP1/2, a DNA-binding tegument protein. Two potential ATP-binding motifs were identified within the predicted amino acid sequence of MDV UL36h. Near the C terminus of the UL36h gene product, 50-60 tandem repeats of two amino acids rich in proline (PX) are found. However, MDV does not contain two leucine zipper motifs found in HSV UL36 encoded protein. Based on the putative function of HSV, MDV UL36 h may also be involved in DNA packaging during viral maturation.