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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #71078
Title: CHARACTERIZATION OF OAT X MAIZE PARTIAL HYBRIDS AND THEIR DERIVATIVES

Author
item RIERA-LIZARAZU, OSCAR - UNIVERSITY OF MINNESOTA
item Rines, Howard
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA

Submitted to: Oat International Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 8/6/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The production of haploids from different Triticeae species and a near total lack of oat (Avena sativa L.) haploid plant recovery from anther culture motivated us to try oat x maize (Zea mays L.) crosses as an alternate means to produce oat haploids. We found that oat x maize crosses not only result in the production of oat haploids but also result in the production of partial hybrids with 21 oat chromosomes plus one to four maize chromosomes. This report describes the characterization of these oat x maize partial hybrids and their progenies. From a series of seven crossing experiments involving cultivated hexaploid oat and maize, we recovered a total of 90 plants through embryo rescue. Fifty-two plants were oat haploids following maize chromosome elimination. Twenty-eight plants were karyotypically stable partial hybrids with one to four maize chromosomes in addition to a haploid set of 21 oat chromosomes. One individual had an oat/maize chromosome translocation. The remaining nine were apparent chromosomal chimeras, where some tissues in a given plant contained maize DNA. Maize DNA probes were used to identify the maize chromosome(s) present in the various oat/maize progenies. Southern analyses using maize RFLP markers revealed that maize chromosomes 2, 3, 4, 5, 6, 7, 8, and 9 were present in partial hybrids and chromosomal chimeras. Karyotypically stable oat/maize derivatives including self-fertile maize chromosome addition oat plants represent unique materials which could be a source for gene transfer from maize to oat. Also, efforts are underway where maize lines carrying Mu transposons have been crossed to oat as an attempt to transfer maize transposons to oat.