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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #71080
Title: MONOSOMICS AND RFLP LINKAGE GROUP ASSOCIATIONS IN OAT

Author
item FOX, STEPHEN - AGRIC./AGRI-FOOD CANADA
item JELLEN, ERIC - KANSAS STATE UNIVERSITY
item KIANIAN, SHAHRYAR - UNIVERSITY OF MINNESOTA
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA
item Rines, Howard

Submitted to: Oat International Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 8/6/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: By using C-banding staining to specifically identify the individual chromosomes, an oat (Avena sativa L. 2n=6x=42) monosomic series has been assembled consisting of monosomic lines obtained from the cultivars 'Kanota', 'Sun II', and from progeny of 'Sun II' oat polyhaploids developed from oat/maize crosses. From these, an F1 monosomic series was developed to associate oat molecular linkage groups with specific oat chromosomes. In a monosomic F1, the presence of both alleles of a polymorphic locus indicates that both homologues of a chromosome carrying that locus are present. The presence of only the paternal form of the polymorphic locus indicates that the maternal chromosome of the homologous pair was not contributed to the zygote and serves to associate the RFLP locus with the monosomic chromosome. Twenty-three linkage groups were associated with 17 different chromosomes using this technique. Four translocations were identified involving chromosome pairs 7C/17, 8/14, K7 and K13 forms of chromosome 14, and K11 and K17 forms of chromosome 17. Comparison of linkage groups and their chromosome associations suggests that intact homoeologous chromosomes in common oat are rare due to substantial numbers of rearrangements. However, these rearrangements may allow novel recombination of traits.