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ARS Home » Research » Publications at this Location » Publication #71635
Title: DISTRIBUTION OF FUMONISINS IN DRY MILLED FRACTIONS OF CONTAMINATED CORN

Author
item Bennett, Glenn
item DENHART, RENE - DEPT AG ENG, U OF IL

Submitted to: Corn Dry Milling Conference
Publication Type: Abstract Only
Publication Acceptance Date: 5/31/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: One lot of fumonisin-contaminated yellow corn and one lot of contaminated white corn were dry milled (in duplicate) using a laboratory scale degerminator. Toxin-free lots of yellow and white corn (<0.5 ug/g FB1) were milled as controls. Samples were tempered, using a two-stage process, to bring the moisture content to 21% before degerminating. After degermination, the throughput was sieved over a <3.5 M screen and the overs put through the degerminator again. The combined throughs were dried and sieved using a Great Western box sifter. Fractions from sieve sizes 3.5, 5, 10, 18, 22, 38, and 66 M were aspirated to separate pericarp. Germ was hand separated from fractions over the 3.5 and 10 M sieves. Material balance data showed that the contaminated yellow corn (10 ug/g FB1) yielded less fine meal, degermer flour, course meal and medium meal than the control lot. Higher yields of flaking grits and pericarp were obtained from the contaminated corn. Contaminated white corn (45 ug/g FB1) yielded less fine meal, degermer flour and grits than the control. Higher yields of germ and pericarp were obtained from the contaminated white corn. Fumonisin assays showed that all dry milled fractions were contaminated with fumonisin B1 and B2. Flaking grits and the course and fine grits accounted for >35% of the total toxins. Preliminary data from a bioassay procedure agreed with HPLC data of selected fractions.