Author
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Hoffman, David |
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Submitted to: Oat International Conference Proceedings
Publication Type: Proceedings Publication Acceptance Date: 7/11/1996 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Maps have been developed for hexaploid and diploid Avena species. The hexaploid map has ten isozyme loci, but just one morphological locus. The diploid maps lack morphological and isozyme loci. Such loci may be simpler alternatives to DNA-based markers for marker-assisted selection and could provide further information for comparative mapping. The objectives of this study were to determine the inheritance of morphological traits and isozyme markers within diploid oats and determine the linkage relationships of the traits and markers found to be monogenically controlled. Crosses were made between two Avena strigosa Schreb. and one A. wiestii Steud., and F2 populations and F3 families were derived for the genetic analysis of six morphological traits and seven putative isozyme loci. An F6 recombinant inbred population was used to check the possibility of linkage of these markers to crown rust resistance loci. The observed phenotypes of each trait or putative locus fit the expectation for monogenic inheritance. One linkage group of five loci was identified. Evidence of linkage conservation between diploid oat, hexaploid oat, and barley was found. Three of the isozyme markers mapped to a linkage group containing the PcA crown rust resistance gene cluster. Acp-1 was found to be 28 cM from PcA. This study of morphological and isozyme genes of diploid oats supplements the molecular mapping effort of the oat genome and provides information toward a more robust genetic map of Avena. |
