Author
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ELIAS, KAROL - NORTH CAROLINA STATE |
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Submitted to: Fungal Genetics Newsletter
Publication Type: Research Notes Publication Acceptance Date: 4/11/1996 Publication Date: N/A Citation: N/A Interpretive Summary: The genus Phomopsis includes diseases of soybeans and other crops. At present species of Phomopsis are identified based on their host but recently it has shown that these fungi are not host-specific. The research tools are needed with which to investigate genetic variability in Phomopsis. This short note reports on the development of mutants in a strain of Phomopsis; this had never been done previously. These mutants can then be used to determine if species of Phomopsis are host-specific and how genetically variable are populations of closely related species of Phomopsis. Technical Abstract: Members of the coelomycete genus Phomopsis have never been assigned to vegetative compatibility groups utilizing nitrate-metabolism mutants in part because of chlorate insensitivity. An isolate of Phomopsis sp. grown on rose bengal amended chlorate medium produced fast growing mutant sectors. The phenotype of each mutant sector was determined by compa aring growth on four differential media containing either nitrate, nitrite, uric acid, or hypoxanthine as sole nitrogen sources. |
