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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #72188
Title: MOLECULAR CHARACTERIZATION OF A CAPRINE ADENOVIRUS

Author
item WILLIAMS, EDWARD - TUSKEGEE UNIVERSITY
item Kleiboeker, Steven
item REDDY, P - TUSKEGEE UNIVERSITY
item Clouser, Deborah
item Lehmkuhl, Howard

Submitted to: Intervirology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/16/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary: Adenoviruses produce respiratory and enteric disease in cattle, sheep, and goats. Two new adenoviruses have been isolated recently from sick goats in the United States. Because current methods to characterize and identify members of the adenovirus family are not always clear cut, the ability to compare these isolates at the molecular level with known characterized isolates will become important. The described unique "fingerprint" produced by adenoviruses at the genetic level can be used to further differentiate adenoviruses. This basic knowledge can also be used by scientists to develop improved diagnostic tools to study adenovirus infections in goats and to provide knowledge needed for vaccine development where the adenovirus could be used to carry the genetic information of another organism inducing an effective immune response.

Technical Abstract: Restriction endonuclease site maps were constructed for the genome of a caprine adenovirus (GAdV), strain NC90-7261, which was isolated in 1990 from a 3-year-old goat with encephalitis. Genomic GAdV DNA was digested with seven restriction endonucleases (RE). Genomic DNA libraries of GAdV were constructed by cloning Bam HI and Hind III restriction fragments into a plasmid vector. Using cloned GADV genomic fragments as probes in Southern blot hybridizations, an RE site map was constructed. The position of several clones was confirmed by limited nucleotide sequencing and the location of several RE sites was determined by single or double RE digestions of cloned fragments. The size of the GAdV genome was determined to be 28.2 Kbp. The restriction pattern described in this report is different from that of other adenoviruses. Although the genomic organization of this GAdV is likely to be similar to that of other adenoviruses, the overall level of sequence similarity is low.