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ARS Home » Southeast Area » Gainesville, Florida » Center for Medical, Agricultural and Veterinary Entomology » Insect Behavior and Biocontrol Research » Research » Publications at this Location » Publication #72300
Title: A NEW HAT TRANSPOSABLE ELEMENT FROM BACTROCERA DORSALIS, HOPPER, IS DISTANTLY RELATED TO HOBO AND AC

Author
item Handler, Alfred - Al
item GOMEZ, SHEILACHU - UNIV. OF FL, GAINESVILLE

Submitted to: Gene
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/26/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary: The ability to achieve gene transfer in economically important insects will depend upon the identification and isolation of new transposable elements that may be developed into gene vectors. In this report scientists at the Center for Medical, Agricultural and Veterinary Entomology, Gainesville, Florida, describe results from experiments which have isolated a new hobo related transposable element, which was named "hopper", from the Oriental fruit fly, Bactrocera dorsalis. Knowledge of the hopper DNA and amino acid sequence will allow the isolation of additional hopper elements as well as new elements from other species. Some of these are expected to be functional, and therefore candidates for gene vector development.

Technical Abstract: A new transposable element from the hobo, Ac, Tam3 transposon family has been isolated as a genomic clone from the Oriental fruit fly, Bactrocera dorsalis. It is approximately 3.1 kb in length with 19 bp inverted terminal repeat sequences having a single mismatch. Though sharing several amino acid sequence identities with other hAT elements, it is distantly related to both hobo and Ac. Among hAT elements identified in insects, it is apparently the most distantly related to hobo.