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Title: ANALYSES OF MONOCLONAL ANTIBODIES REACTING WITH PORCINE CD5: RESULTS OF THESECOND INTERNATIONAL SWINE CD WORKSHOP

Author
item PESCOVITZ, M - INDIANA UNIVERSITY
item BOOK, B - INDIANA UNIVERSITY
item AASTED, B - ROYAL VET & AGRI UNIV
item DOMINGUEZ, J - INIA
item BULLIDO, R - INIA
item TREBICHAVSKY, I - INST MICROBIOOGY
item NOVIKOV, B - ALL-RUSSIAN RIVVM
item VALPOTIC, I - UNIVERSITY OF ZAGREB
item Lunney, Joan
item Boyd, Patricia

Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/12/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary: During the Second International Swine CD Workshop two new anti-CD5 monoclonal antibodies (mAbs) were identified. These mAbs, 1H6/8 and BB6-9G12, reacted similarly to the standard CD5 mAb, b53b7; thus, they differentiate between subsets of T lymphocytes, those which kill virus infected cells and the T cell subsets that acts as a natural killer cell, killing tumor cell lines. They also will help distinguish between subsets of mature and immature B cells. Unfortunately neither of these mAb recognized a new determinant on the CD5 molecule. The international workshop, sponsored by the International Union of Immunological Scientists, enables scientists worldwide to independently verify the reactivity of panels of mAb and thus to precisely identify important new reagents to aid analyses of swine immune responses, particularly those involved in controlling disease and vaccine responses. Indeed, these new anti-wCD5 mAb will further aid in studies determining the complexity of swine cytotoxic T cells as well as distinguishing swine B cell subsets.

Technical Abstract: Among the 57 monoclonal antibodies analyzed within the T-cell group, three mAb fell within cluster T13 including the CD5a standard b537b (No. 174). The two new mAb 1H6/8 (No. 058) and BB6- 9G12 (No. 166) both precipitated 55 and 60 kDa proteins that were of similar molecular weights as the standard. Staining patterns on the various cell types were similar. Both new antibodies inhibited the binding of the CD5a reference mAb b537b to peripheral lymphocytes. These mAb therefore both react with the CD5a epitope bringing the number of anti-porcine CD5 mAb to 8, all of which appear to recognize the same epitope.