INCREASE OF GLYCOCALYX AND ALTERED LECTIN AGGLUTINATION PROFILES OF PASTEURELLA HEMOLYTICA A1 INCUBATED IN BOVINE TISSUE CHAMBERS IN VIVO OR INRUMINANT SERUM IN VITRO (HAP 1996 INT. CONF., ACAPULCO, MEXICO)

Authors: BROGDEN, KIM; CLARKE, C - OKU, STILLWATER, OKLAHOMA

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/15/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: P. haemolytica A1 was incubated in bovine subcutaneous tissue chambers and heated ovine or bovine serum (also treated with protein G to remove gamma globulin) to assess changes in morphology and lectin agglutination profiles. Cells, removed from chambers after 2 h, were covered with an extensive, dense glycocalyx extending 0.5 um from the cell. Cells, removed at 6 h, had a sparse glycocalyx of fine fibers 0.2 to 0.3 um from the cell. P. haemolytica incubated for 2 h in ovine or bovine serum were also covered with a sparse glycocalyx. The glycocalyx did not bind Protein A colloidal gold and therefore did not contain aggregates of accumulated antibody. This surface, induced after 2 h incubation in ruminant serum, altered lectin agglutination profiles indicating that some surface carbohydrate moieties were masked by serum components possibly absorbed to the cell.