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Title: VIRUS SUSCEPTIBILITIES OF NEW CONTINUOUS CELL LINES FROM EMBRYOS OF THE WHITEMARKED TUSSOCK MOTH, ORGYIA LEUCOSTIGMA

Author
item LYNN, DWIGHT
item SHAPIRO, MARTIN

Submitted to: In Vitro Cellular And Developmental Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/14/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary: The whitemarked tussock moth is a common pest of deciduous trees in North America, often causing severe defoliation of shade and fruit trees. Some viruses which infect this insect have potential for use as biological controls of these pests. This research developed and described three new cell lines from the whitemarked tussock moth. These cell lines are susceptible to several viruses and will be useful in the study of these pathogens. Ultimately, one or more of the cell lines could be used for mass producing the virus(es) for use as biological pesticides leading to reduced reliance on chemical control methods.

Technical Abstract: Three new cell lines were developed from embryos of the whitemarked tussock moth, Orgyia leucostigma. The lines have different growth patterns and are morphologically distinct from each other. Isoenzyme analysis shows they are distinct from all other cells maintained in the laboratory. The lines have been designated IPLB-OlE505s, IPLB-OlE505A and IPLB-OlE-7. Inoculation with several viruses show they are susceptible to their homologous nuclear polyhedrosis virus (NPV) and cytoplasmic polyhedrosis virus (CPV), an NPV from a related species, Orgyia pseudotsugata and a CPV isolated from Lymantria dispar. They do not appear to be susceptible to any of several noctuid NPVs nor a CPV from Trichoplusia ni.