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ARS Home » Research » Publications at this Location » Publication #73927

Title: OPTIMAL SEPARATION OF JOJOBA PROTEIN USING MEMBRANE PROCESSES

Author
item NABETANI, HIROSHI - NATL FOOD RES INST
item Abbott Dr, Thomas
item KLEIMAN, ROBERT - RETIRED, USDA/ARS/NCAUR
item NAKAJIMA, MITSUTOSHI - NATL FOOD RES INST

Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: 6/1/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The efficiency of a pilot-scale membrane system for purifying and concentrating jojoba protein was estimated. In this system, a jojoba extract was first clarified with a microfiltration membrane. The clarified extract was diafiltrated and the protein was purified with an ultrafiltration membrane. Then the protein solution was concentrated with the ultrafiltration membrane. Permeate flux during microfiltration was essentially independent of solids concentration in the feed, in contrast with the permeate flux during ultrafiltration which was a function of protein concentration. Based on these results, a mathematical model which describes the batch-wise concentration process with ultrafiltration membranes was developed. Using this model, the combination of batch-wise concentration with diafiltration was optimized, and an industrial scale process was designed. The effect of EDTA on the performance of the membrane system was also investigated. The addition of EDTA increased the concentration of protein in the extract and improved the recovery of protein in the final products. The quality of the final product (color and solubility) was also improved. However, EDTA decreased permeate flux during ultrafiltration.