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ARS Home » Research » Publications at this Location » Publication #74119
Title: PANCREATIC ENZYME AND CHOLECYSTOKININ RESPONSE TO ABOMASALLY INFUSED CASEININ THE SHEEP

Author
item WALDNER, D. - UNIV OF KENTUCKY
item ELY, D. - UNIV OF KENTUCKY
item HARMON, D. - UNIV OF KENTUCKY
item McLeod, Kyle
item AARON, D. - UNIV OF KENTUCKY

Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: 7/24/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Eight crossbred wethers (30 kg BW), with T-shaped intestinal cannulas and abomasal infusion catheters, were used in a 4x8 Latin rectangle to determine the influence of small intestine (SI) protein availability on the activity of selected enzymes in intestinal digesta and cholecystokinin (CCK) release. Animals were maintained on a 10% CP diet with intake limited to 2% BW. Treatments, infused abomasally (50 mL/h), were a control (water) or a solution providing 1, 2 or 3 g casein / (kg BW * d). Each experimental period lasted 16 d, the first 8 d serving as an adaptation period. Nitrogen balance was determined on d 9 to 12, followed by intestinal digesta sampling on d 13 to 15, and jugular blood collection on d 16. Digesta samples (50 mL) were taken 4 times per d at 6-h intervals with an 8-h interval between days. Blood samples were taken over a 24-h period at 4-h intervals. Nitrogen digestibility and daily N retained (% of N digested) increased linearly (P < .01) with increasing SI protein availability (casein). Specific and total activity of amylase and chymotrypsin in intestinal digesta increased linearly (P < .01), whereas trypsin showed a quadratic response (P < .01) to casein infusion. Release of CCK was not different among treatments. The amount of protein available for digestion in the SI has a positive impact on digestive enzyme activity. However, the pattern on the response for each enzyme may differ and does not appear to be related to long term CCK release.