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Title: GLIADIN BLOCK ANALYSIS BY RP-HPLC AND CAPILLARY ELECTROPHORESIS FOR GENOTYPE IDENTIFICATION AND QUALITY PREDICTION

Author
item Bietz, Jerold
item WAGA, J - PLANT BREED INST - POLAND

Submitted to: Cereal Conference Royal Australian Chemical Institute Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 9/4/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Gliadins are coded by genes at six complex loci on wheat chromosome groups 1 and 6. Genes in each locus are closely linked, causing co-expression of proteins they code. Alleles or "blocks" differ among varieties, however, so genotypes can be represented by formulae identifying alleles at each locus [Sozinov and Poperelya, Ann. Technol. Agric. 29:229-245 (1980)]. Gliadin blocks are often markers of quality characteristics, so allele analysis may be valuable for selection during breeding, and for genotype identification. Gel electrophoresis has been most used to identify gliadin blocks, but reversed-phase high-performance liquid chromatography (RP-HPLC) and capillary electrophoresis (CE) now offer advantages for gliadin analysis. We therefore used gel electrophoresis, RP-HPLC, and CE to analyze wheat segregants from a cross of varieties Alcedo and Trakija, differing by single gliadin alleles. Each method revealed compositions, genetic segregation, and recombination of gliadin alleles, useful for quality selection during breeding. HPLC and CE, because of their excellent resolution, also revealed some gliadins not apparent by gel electrophoresis. Such information may help extend current knowledge of gliadin alleles. Block analyses by RP-HPLC and CE can become valuable methods, complementary to gel electrophoresis, during wheat breeding and in genetic studies.