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ARS Home » Research » Publications at this Location » Publication #74833
Title: AUTOMATED SIZING OF FLUORESCENT LABELED SIMPLE SEQUENCE REPEAT (SSR) MARKERS FOR SOYBEAN GENOTYPE IDENTIFICATION

Author
item DIWAN, NOA - UNIVERSITY OF MARYLAND
item Cregan, Perry

Submitted to: Plant Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 8/29/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The classification of eukaryotic genomes based upon SSR allele sizing provides a useful tool for estimating genetic distance between organisms and for genotype identification. The objective of this work was to 1) establish a system to quantify genetic variability in soybean using allelic frequencies of 20 SSR loci in 35 soybean genotypes that account for greater than 95% of the alleles in N. American soybean cultivars; and 2) determine if this system can be used to distinguish modern cultivars with identical morphological, pigmentation, and other traits commonly used for purposes of variety identification. Sizing of alleles present in the 35 genotypes using fluorescent labeled PCR products analyzed on a Perkin-Elmer ABI Automated DNA Sequencer indicated an average of 10.1 alleles per locus (range: 7 to 15). Mean gene diversity of the 20 loci was 0.79 (range: 0.68 to 0.90). Maturity group I, II, IV and VI cultivars identical for standard morphological, pigmentation, etc. traits were selected from the Plant Variety Protection Office database and were easily distinguished using allelic profiles of the 20 SSR loci.