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ARS Home » Research » Publications at this Location » Publication #74904
Title: MINISATELLITES AND SSR DNA MARKERS IN AVOCADO

Author
item LAVI, URI - ISRAEL
item SHARON, DROR - ISRAEL
item MHAMEED, SIVH - ISRAEL
item LAHAV, EMIL - ISRAEL
item HILLEL, JOSSI - ISRAEL
item CREGAN, PERRY

Submitted to: Plant Genome Analysis Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 8/29/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Multi-locus probes reveal individual specific band patterns in avocado. The level of heterozygosity at these loci is 94%-100%. Mendelian inheritance is found at most loci. The patterns of the three avocado races and nine Persea species were analyzed to determine genetic relationships. Linkage analysis in the progeny of a cross of the avocado o cultivars Pinkerton x Ettinger revealed the Pinkerton band p8 to be associated with purple fruit skin color. This result was verified in the progeny of Pinkerton x Bacon. We interpreted this as genetic linkage between the p8 band and i locus which regulates fruit skin color. Simple Sequence Repeat (SSR) DNA markers have been generated in avocado by screening 10,000 clones from a genomic library with various simple sequence repeat probes. Sixty-six loci were available for analysis. These had an average of 3.4 alleles per locus. The mean gene diversity of the 66 loci was 0.52 and the mean heterozygosity level was 62%. Fift of the SSR loci were polymorphic and informative in the analysis of 50 progeny of the cross of Pinkerton x Ettinger. The SSR markers were mostly inherited in a Mendelian fashion. The resulting genetic map contained 10 linkage groups having 2-5 markers in each and covering over 400 cM.