Author
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Cregan, Perry |
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Tamulonis, John |
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Submitted to: International Treiticeae Mapping Initiative Workshop
Publication Type: Abstract Only Publication Acceptance Date: 8/29/1996 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: The goal of our collaborative soybean microsatellite (SSR) development project is the identification of 640 single locus markers that are polymorphic in adapted soybean germplasm. The steps in this process are genomic library construction and screening, sequence determination and analysis, PCR primer selection, and primer analysis and marker characterization. Each of these steps has been examined to maximize the proportion of SSR-containing genomic clones ultimately converted into "clean", single locus markers that are polymorphic in cultivated soybean. Particular emphasis has been placed on systems to create genomic libraries that optimize genome sampling; selection of the core motif(s) that gives the highest proportion of single locus, maximally polymorphic markers; PCR primer selection criteria to improve primer success; and primer screening and characterization techniques that maximize throughput. In soybean it has been possible to significantly increase the rate of microsatellite marker development. This has been the result of 1) isolating only tri-nucleotide microsatellites, 2) optimizing primer selection, and 3) increasing throughput in primer testing and characterization. Our initial experience with wheat microsatellite markers, coupled with available data relating to levels of polymorphism of various microsatellite core motifs, suggest that similar improvements in the efficiency of wheat microsatellite development can be obtained. |
