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Title: REGISTRATION OF FOUR PAIRS OF SOYBEAN NEAR-ISOGENIC LINES, BARC-14 TO BARC-17, NODULATED VS. NON-NODULATED.

Author
item LEFFEL, ROBERT - RETIRED
item YAKLICH, ROBERT

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/1/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary: The world demand for animal products is increasing as developing nations include more meat in their diets. Soybeans provide a stable, consistent, source of protein meal for animal diets. Soybean plants obtain their nitrogen from soil mineralization and from atmospheric nitrogen. The atmospheric nitrogen is converted to a usable form by symbiotic bacteria that live in nodules that form on the soybean root surface. In order for scientists to study nitrogen and protein metabolism in soybean, a non- nodulating genetic character was bred into two high-protein lines and two commercial cultivars. These four germplasm lines are being released for public use to facilitate the study of nitrogen nutrition in the soybean plant. These germplasm lines will allow plant scientists to separately study the contribution that nitrogen from the soil and from the nodule bacteria make to the nitrogen, protein and amino acid metabolism of the soybean plant.

Technical Abstract: Soybean plants obtain their nitrogen from soil mineralization and atmospheric nitrogen. The latter is converted to a useable form by symbiotic bacteria in nodules on the surface of the root. The objective of this study was to form near-isogenic lines of soybean genotypes that did not fix atmospheric nitrogen. The pairs of near-isogenic lines were derived from crosses between `Clark rj1' and the recurrent parent high protein lines D76-8070 and CX797-21 and the determinate cultivars `Essex' and `Ripley'. The isolines were developed by maintaining heterozygosity at the RJ1 locus while inbreeding occurred at all other loci. The recurrent parents were backcrossed to the F1 plants and after two more backcross generations, single seed from the BC3 generation were planted in hill plots. Seed from the single plants of the BC3S1 were planted in progeny rows the following year. Subsequent cycles consisted of selfing and selecting single plants from segregating rows to plant in individual plant rows the following year. In 1995, progeny rows were selected from the BC3S6 generation based on homozygosity at the RJ1, rj1 locus. These lines are being released for experimental use for basic research in nitrogen, protein, and amino acid metabolism in soybean.