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Title: DESIGNS OF REFERENCE FAMILIES FOR GENE MAPPING IN DOMESTIC ANIMALS

Author
item Da, Yang
item Vanraden, Paul
item LI, NING - CHINA AGRIC UNIV, BEIJING
item WU, CHANGXIN - CHINA AGRIC UNIV, BEIJING

Submitted to: Plant Genome Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 1/11/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The reference family panel is the foundation of a gene mapping program because it affects the cost and quality of the genetic linkage maps. An optimum design should be one that minimizes the gene mapping cost for the same quality of linkage maps. A map cost function was defined as the number of genotypes required per unit of map coverage and was used to determine the optimum detection level and to measure the efficiency of each design. For different designs, the optimum detection levels of recombination frequency that minimizes the map cost were found to be in the neighborhood of 0.11-0.15. As the family size increases, the optimum detection level increases and the map cost decreases. Given small detection levels, however, small families have slight advantage over large families. Substantial reduction in map cost can be achieved by using highly polymorphic markers. Grandparents are important for small families in the reduction of map cost and become less important as the family size increases. Assuming equal recombination frequency in adjacent marker intervals, the optimum level of recombination frequency for ordering loci was found to be around 0.12, which overlaps the optimum detection level. However, ordering loci would require more genotypes than detecting linkage. To order closely linked loci, much larger sample size would be required. Nine designs applicable to domestic animals were described with seven three-generation and two two-generation families or six full-sib and three half-sib families. Sample size requirements were derived for six representative designs.