Author
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Ahlgren, Jeffrey |
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Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only Publication Acceptance Date: 11/26/1996 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: A new commercially available electrophoretic gel system that utilizes a neutral pH was used to characterize glucansucrases from several Leuconostoc mesenteroides species. Following electrophoretic separation, whole gels or portions of them could be incubated in sucrose solutions which resulted in the biosynthesis of a new polysaccharide within the gel matrix. When compared to the standard Laemlli style Tris-buffered SDS-PAGE system, the neutral pH system was significantly more sensitive and yielded an enhanced signal when stained for carbohydrate with a periodic acid/Schiff reagent method. Both alternansucrase and dextransucrase activity could be measured in neutral pH-buffered gels. The system is also useful for separating proteins prior to western blotting for protein microsequence analysis; i.e., after electrophoresis and western blotting onto PVDF membranes, the membrane could be incubated in sucrose and de novo polysaccharide biosynthesis detected. |
