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ARS Home » Plains Area » Grand Forks, North Dakota » Grand Forks Human Nutrition Research Center » Dietary Prevention of Obesity-related Disease Research » Research » Publications at this Location » Publication #77221
Title: DIETARY COPPER DEFICIENCY REDUCES NEUTROPHIL ADHERENCE IN VIVO

Author
item SCHUSCHKE, D - UNIVERSITY OF LOUISVILLE
item Saari, Jack
item MILLER, F - UNIVERSITY OF LOUISVILLE

Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 4/6/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Dietary copper deficiency depresses the copper status and functional activities of neutrophils. In the current study, neutrophilendothelium adhesion in response to the chemotactic peptide N/formyl-methionyl-leucyl -phenylalanine (fMLP) was studied. Male weanling Sprague-Dawley rats were fed purified diets that were either copper-adequate (CuA, 6 ug Cu/g) or copper-deficient (CuD, 0.4 ug (Cu/g) for 4 weeks. Rats were anesthetized and the cremaster muscle was prepared for in vivo videomicroscopy. Intravascular rolling and sticking of leukocytes were counted for 5 min periods before and every 10 min for 60 min after the topical administration of 10**-7 M fMLP. Results are % of baseline values. Group n Rollers Stickers CuA 7 18.7 +/- 5.0 147.0 +/- 32.6 CuD 6 17.5 +/- 2.6 57.3 +/- 4.1* Values are mean +/- SEM, *p<0.05 compared to CuA. These results demonstrate that leukocyte-endothelial cell behavior is altered by copper restriction. The results suggest decreased ICAM binding which mediates leukocyte adherence. The depressed leukocyte adherence may have a role in the decreased neutrophil activity found in copper deficiency. Supported by USDA Agreement 95-37200-1625.